Analysis of Anthocyanin Composition and Content Contained from Grains of the Korean Purple Rice Varieties by Liquid Chromatography with Diode Array Detection and Electrospray Ionization/Mass Spectrometry(LC-DAD-ESI/MS): LC-DAD-ESI/MS를 이용한 국내 자색벼 품종 종실의 안토시아닌 조성 및 함량 분석

The results of evaluating the individual anthocyanin composition and content of domestic purple rice varieties using LC-DAD-ESI/MS are as follows.
1. By confirming the MS fragment patterns of the chemical structures of all individual anthocyanins isolated from purple rice varieties, a total of four individual components were isolated and identified, including one unknown compound based on cyanidin, cyanidin 3,5-diglucoside, cyanidin 3-glucoside, and peonidin 3-glucoside.
2. Cyanidin 3-glucoside and peonidin 3-glucoside were the main components, with cyanidin 3-glucoside showing the highest content proportion of over 90%, and the average content of individual components was in the order of cyanidin 3-glucoside > peonidin 3-glucoside > cyanidin 3,5-diglucoside > unknown (cyanidin based).
3. The total anthocyanin content of Heukjinju rice was 408 mg/100 g, which is about 6 times higher than that of Heuknam rice.


The content and composition of anthocyanin were determined in grains of the Korean purple rice varieties using liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (LC-DAD-ESI/MS). Isolated individual anthocyanins were identified and confirmed as cyanidin 3,5-diglucoside, cyanidin 3-glucoside, peonidin 3-glucoside and unknown compound with cyanidin based by interpretation MS fragments. Among them cyanidin 3-glucoside and peonidin 3-glucoside were major components. Especially, cyanidin 3-glucoside showed highest proportion and the anthocyanin contents were found to be significantly higher in Heugjinjubyeo(408 mg/100 g) than in other variety.