Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley

Linsan Liu; Sarah B. Jose; Chiara Campoli; Micha M. Bayer; Miguel A. Sánchez-Diaz; Trisha McAllister; Yichun Zhou; Mhmoud Eskan; Linda Milne; Miriam Schreiber; Thomas Batstone; Ian D. Bull; Luke Ramsay; Penny von Wettstein-Knowles; Robbie Waugh; Alistair M. Hetherington; Sarah M. McKim

doi:10.1038/s41467-022-33300-1

Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley

Linsan Liu
, Sarah B. Jose
, Chiara Campoli
, Micha M. Bayer
, Miguel A. Sánchez-Diaz
, Trisha McAllister
, Yichun Zhou
, Mhmoud Eskan
, Linda Milne
, Miriam Schreiber
, Thomas Batstone
, Ian D. Bull
, Luke Ramsay
, Penny von Wettstein-Knowles
, Robbie Waugh
, Alistair M. Hetherington
, Sarah M. McKim

University of Dundee

Research output: Contribution to journal › Article › peer-review

Abstract

AbstractFaced with terrestrial threats, land plants seal their aerial surfaces with a lipid-rich cuticle. To breathe, plants interrupt their cuticles with adjustable epidermal pores, called stomata, that regulate gas exchange, and develop other specialised epidermal cells such as defensive hairs. Mechanisms coordinating epidermal features remain poorly understood. Addressing this, we studied two loci whose allelic variation causes both cuticular wax-deficiency and misarranged stomata in barley, identifying the underlying genes, Cer-g/ HvYDA1, encoding a YODA-like (YDA) MAPKKK, and Cer-s/ HvBRX-Solo, encoding a single BREVIS-RADIX (BRX) domain protein. Both genes control cuticular integrity, the spacing and identity of epidermal cells, and barley’s distinctive epicuticular wax blooms, as well as stomatal patterning in elevated CO2 conditions. Genetic analyses revealed epistatic and modifying relationships between HvYDA1 and HvBRX-Solo, intimating that their products participate in interacting pathway(s) linking epidermal patterning with cuticular properties in barley. This may represent a mechanism for coordinating multiple adaptive features of the land plant epidermis in a cultivated cereal.

Original language	English
Journal	Nature Communications
DOIs	https://doi.org/10.1038/s41467-022-33300-1
Publication status	Published - 13 Oct 2022

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Liu, L., Jose, S. B., Campoli, C., Bayer, M. M., Sánchez-Diaz, M. A., McAllister, T., Zhou, Y., Eskan, M., Milne, L., Schreiber, M., Batstone, T., Bull, I. D., Ramsay, L., von Wettstein-Knowles, P., Waugh, R., Hetherington, A. M., & McKim, S. M. (2022). Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley. Nature Communications. https://doi.org/10.1038/s41467-022-33300-1

@article{d9fb2706e02e40a0972a8b4be80bab56,

title = "Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley",

abstract = "AbstractFaced with terrestrial threats, land plants seal their aerial surfaces with a lipid-rich cuticle. To breathe, plants interrupt their cuticles with adjustable epidermal pores, called stomata, that regulate gas exchange, and develop other specialised epidermal cells such as defensive hairs. Mechanisms coordinating epidermal features remain poorly understood. Addressing this, we studied two loci whose allelic variation causes both cuticular wax-deficiency and misarranged stomata in barley, identifying the underlying genes, Cer-g/ HvYDA1, encoding a YODA-like (YDA) MAPKKK, and Cer-s/ HvBRX-Solo, encoding a single BREVIS-RADIX (BRX) domain protein. Both genes control cuticular integrity, the spacing and identity of epidermal cells, and barley{\textquoteright}s distinctive epicuticular wax blooms, as well as stomatal patterning in elevated CO2 conditions. Genetic analyses revealed epistatic and modifying relationships between HvYDA1 and HvBRX-Solo, intimating that their products participate in interacting pathway(s) linking epidermal patterning with cuticular properties in barley. This may represent a mechanism for coordinating multiple adaptive features of the land plant epidermis in a cultivated cereal.",

author = "Linsan Liu and Jose, \{Sarah B.\} and Chiara Campoli and Bayer, \{Micha M.\} and S{\'a}nchez-Diaz, \{Miguel A.\} and Trisha McAllister and Yichun Zhou and Mhmoud Eskan and Linda Milne and Miriam Schreiber and Thomas Batstone and Bull, \{Ian D.\} and Luke Ramsay and \{von Wettstein-Knowles\}, Penny and Robbie Waugh and Hetherington, \{Alistair M.\} and McKim, \{Sarah M.\}",

year = "2022",

month = oct,

day = "13",

doi = "10.1038/s41467-022-33300-1",

language = "English",

journal = "Nature Communications",

issn = "2041-1723",

}

Liu, L, Jose, SB, Campoli, C , Bayer, MM, Sánchez-Diaz, MA, McAllister, T, Zhou, Y, Eskan, M, Milne, L , Schreiber, M, Batstone, T, Bull, ID, Ramsay, L, von Wettstein-Knowles, P, Waugh, R, Hetherington, AM & McKim, SM 2022, 'Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley', Nature Communications. https://doi.org/10.1038/s41467-022-33300-1

TY - JOUR

T1 - Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley

AU - Liu, Linsan

AU - Jose, Sarah B.

AU - Campoli, Chiara

AU - Bayer, Micha M.

AU - Sánchez-Diaz, Miguel A.

AU - McAllister, Trisha

AU - Zhou, Yichun

AU - Eskan, Mhmoud

AU - Milne, Linda

AU - Schreiber, Miriam

AU - Batstone, Thomas

AU - Bull, Ian D.

AU - Ramsay, Luke

AU - von Wettstein-Knowles, Penny

AU - Waugh, Robbie

AU - Hetherington, Alistair M.

AU - McKim, Sarah M.

PY - 2022/10/13

Y1 - 2022/10/13

N2 - AbstractFaced with terrestrial threats, land plants seal their aerial surfaces with a lipid-rich cuticle. To breathe, plants interrupt their cuticles with adjustable epidermal pores, called stomata, that regulate gas exchange, and develop other specialised epidermal cells such as defensive hairs. Mechanisms coordinating epidermal features remain poorly understood. Addressing this, we studied two loci whose allelic variation causes both cuticular wax-deficiency and misarranged stomata in barley, identifying the underlying genes, Cer-g/ HvYDA1, encoding a YODA-like (YDA) MAPKKK, and Cer-s/ HvBRX-Solo, encoding a single BREVIS-RADIX (BRX) domain protein. Both genes control cuticular integrity, the spacing and identity of epidermal cells, and barley’s distinctive epicuticular wax blooms, as well as stomatal patterning in elevated CO2 conditions. Genetic analyses revealed epistatic and modifying relationships between HvYDA1 and HvBRX-Solo, intimating that their products participate in interacting pathway(s) linking epidermal patterning with cuticular properties in barley. This may represent a mechanism for coordinating multiple adaptive features of the land plant epidermis in a cultivated cereal.

AB - AbstractFaced with terrestrial threats, land plants seal their aerial surfaces with a lipid-rich cuticle. To breathe, plants interrupt their cuticles with adjustable epidermal pores, called stomata, that regulate gas exchange, and develop other specialised epidermal cells such as defensive hairs. Mechanisms coordinating epidermal features remain poorly understood. Addressing this, we studied two loci whose allelic variation causes both cuticular wax-deficiency and misarranged stomata in barley, identifying the underlying genes, Cer-g/ HvYDA1, encoding a YODA-like (YDA) MAPKKK, and Cer-s/ HvBRX-Solo, encoding a single BREVIS-RADIX (BRX) domain protein. Both genes control cuticular integrity, the spacing and identity of epidermal cells, and barley’s distinctive epicuticular wax blooms, as well as stomatal patterning in elevated CO2 conditions. Genetic analyses revealed epistatic and modifying relationships between HvYDA1 and HvBRX-Solo, intimating that their products participate in interacting pathway(s) linking epidermal patterning with cuticular properties in barley. This may represent a mechanism for coordinating multiple adaptive features of the land plant epidermis in a cultivated cereal.

UR - http://dx.doi.org/10.1038/s41467-022-33300-1

U2 - 10.1038/s41467-022-33300-1

DO - 10.1038/s41467-022-33300-1

M3 - Article

SN - 2041-1723

JO - Nature Communications

JF - Nature Communications

ER -

Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley

Abstract

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