Identification of the phenolic components in Bulgarian raspberry cultivars by LC-ESI-MSn.

Derek Stewart; I. Dincheva; I. Badjakov; V. Kondakova; Pat Dobson; Gordon McDougall

Identification of the phenolic components in Bulgarian raspberry cultivars by LC-ESI-MSn.

Derek Stewart
, I. Dincheva
, I. Badjakov
, V. Kondakova
, Pat Dobson
, Gordon McDougall

Plant adaptations, biochemistry and food quality

AgroBioInstitute

Research output: Contribution to journal › Article › peer-review

Abstract

A high-performance liquid chromatography (HPLC) method coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive and negative ion modes has been used to identify phenolic compounds in five red raspberry cultivars from Bulgaria. Photodiode-array detection (PDA) has been used to screen the main classes of phenolic compounds, such as ellagitannins, anthocyanins, flavonol glucosides, flavan-3-ols and phenolic acid glycosides. A total of 60 components were tentatively characterized. The anthocyanins were cyanidin and pelargonidin types, found predominantly as their glucosylrutinosides. The major flavonol glucosides were quercetin and kaempferol types found mainly as their rutinosides and glucuronides. Antioxidant activity, total phenolic content and total anthocyanin content were evaluated by FRAP assay, Folin-Ciocalteu method and pH-differential method respectively.

Original language	English
Pages (from-to)	127-137
Number of pages	11
Journal	International Journal of Agricultural Science and Research (IJASR),
Volume	3
Issue number	3
Publication status	Published - 1 Aug 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Raspberry
Bulgarian
LC-MS/MS

Cite this

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title = "Identification of the phenolic components in Bulgarian raspberry cultivars by LC-ESI-MSn.",

abstract = "A high-performance liquid chromatography (HPLC) method coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive and negative ion modes has been used to identify phenolic compounds in five red raspberry cultivars from Bulgaria. Photodiode-array detection (PDA) has been used to screen the main classes of phenolic compounds, such as ellagitannins, anthocyanins, flavonol glucosides, flavan-3-ols and phenolic acid glycosides. A total of 60 components were tentatively characterized. The anthocyanins were cyanidin and pelargonidin types, found predominantly as their glucosylrutinosides. The major flavonol glucosides were quercetin and kaempferol types found mainly as their rutinosides and glucuronides. Antioxidant activity, total phenolic content and total anthocyanin content were evaluated by FRAP assay, Folin-Ciocalteu method and pH-differential method respectively.",

keywords = "Raspberry, Bulgarian, LC-MS/MS",

author = "Derek Stewart and I. Dincheva and I. Badjakov and V. Kondakova and Pat Dobson and Gordon McDougall",

year = "2013",

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TY - JOUR

T1 - Identification of the phenolic components in Bulgarian raspberry cultivars by LC-ESI-MSn.

AU - Stewart, Derek

AU - Dincheva, I.

AU - Badjakov, I.

AU - Kondakova, V.

AU - Dobson, Pat

AU - McDougall, Gordon

PY - 2013/8/1

Y1 - 2013/8/1

N2 - A high-performance liquid chromatography (HPLC) method coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive and negative ion modes has been used to identify phenolic compounds in five red raspberry cultivars from Bulgaria. Photodiode-array detection (PDA) has been used to screen the main classes of phenolic compounds, such as ellagitannins, anthocyanins, flavonol glucosides, flavan-3-ols and phenolic acid glycosides. A total of 60 components were tentatively characterized. The anthocyanins were cyanidin and pelargonidin types, found predominantly as their glucosylrutinosides. The major flavonol glucosides were quercetin and kaempferol types found mainly as their rutinosides and glucuronides. Antioxidant activity, total phenolic content and total anthocyanin content were evaluated by FRAP assay, Folin-Ciocalteu method and pH-differential method respectively.

AB - A high-performance liquid chromatography (HPLC) method coupled with electrospray ionization mass spectrometric (ESI-MS) detection in positive and negative ion modes has been used to identify phenolic compounds in five red raspberry cultivars from Bulgaria. Photodiode-array detection (PDA) has been used to screen the main classes of phenolic compounds, such as ellagitannins, anthocyanins, flavonol glucosides, flavan-3-ols and phenolic acid glycosides. A total of 60 components were tentatively characterized. The anthocyanins were cyanidin and pelargonidin types, found predominantly as their glucosylrutinosides. The major flavonol glucosides were quercetin and kaempferol types found mainly as their rutinosides and glucuronides. Antioxidant activity, total phenolic content and total anthocyanin content were evaluated by FRAP assay, Folin-Ciocalteu method and pH-differential method respectively.

KW - Raspberry

KW - Bulgarian

KW - LC-MS/MS

UR - https://www.cabidigitallibrary.org/doi/full/10.5555/20133297877

M3 - Article

SN - 2250-0057

VL - 3

SP - 127

EP - 137

JO - International Journal of Agricultural Science and Research (IJASR),

JF - International Journal of Agricultural Science and Research (IJASR),

IS - 3

ER -

Identification of the phenolic components in Bulgarian raspberry cultivars by LC-ESI-MSn.

Abstract

UN SDGs

Keywords

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