Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay

S. Mitchell; P. Dobson; H. V. Davies; R. Viola

doi:10.1007/BF02358970

Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay

S. Mitchell
, P. Dobson
, H. V. Davies
, R. Viola

Research output: Contribution to journal › Article › peer-review

Abstract

Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.

Original language	English
Pages (from-to)	383-386
Number of pages	4
Journal	Potato Research
Volume	41
Issue number	4
DOIs	https://doi.org/10.1007/BF02358970
Publication status	Published - 1998

Keywords

Alcohol dehydrogenase
Phosphoglucose isomerase
Solanum tuberosum L.
Sugar assay

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10.1007/BF02358970

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@article{b50e4207ef6048aba0d957354d3f0443,

title = "Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay",

abstract = "Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.",

keywords = "Alcohol dehydrogenase, Phosphoglucose isomerase, Solanum tuberosum L., Sugar assay",

author = "S. Mitchell and P. Dobson and Davies, \{H. V.\} and R. Viola",

year = "1998",

doi = "10.1007/BF02358970",

language = "English",

volume = "41",

pages = "383--386",

journal = "Potato Research",

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T1 - Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay

AU - Mitchell, S.

AU - Dobson, P.

AU - Davies, H. V.

AU - Viola, R.

PY - 1998

Y1 - 1998

N2 - Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.

AB - Artefactual results were obtained during assay of sugars in ethanolic extracts of potato tubers with the microplate method. The problem was attributed to contamination with alcohol dehydrogenase of the commercial (yeast) phosphoglucose isomerase used in the assay. The use of phosphoglucose isomerase from another source (rabbit muscle) eliminated the problem.

KW - Alcohol dehydrogenase

KW - Phosphoglucose isomerase

KW - Solanum tuberosum L.

KW - Sugar assay

UR - https://www.mendeley.com/catalogue/a68e85dc-bacd-3c92-b6cd-563881d82151/

U2 - 10.1007/BF02358970

DO - 10.1007/BF02358970

M3 - Article

VL - 41

SP - 383

EP - 386

JO - Potato Research

JF - Potato Research

IS - 4

ER -

Potential for artefacts in the measurement of fructose and sucrose in extracts of potato tubers using the microplate reader assay

Abstract

Keywords

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